Etude in vitro des pouvoirs antithrombotique et antioxydant du Séné (Cassia angustifolia vahl).

Abstract

cassia angustifolia vahl , belonging to Fabaceae Family, locally named « Senna makki », is a plant widely used for medicinal purposes in all four corners of the world.The present study aims to assess the antioxidant and antithrombotic activities of phenolic extracts of the Cassia angustifolia vahl plant and their phytochemical characterization.The determination of the yield of crude extracts from the leaves of Cassia angustifolia proved that the yields vary considerably according to the extraction solvent (EtOH or decocted), of which the yield is greater in the ethanolic extract (19%).Qualitative analysis of Cassia angustifolia leaf extracts by phytochemical screening revealed the existence of many constituents, including alkaloids, polyphenols, flavonoids and tannins. While the quantitative analysis shows that the decocted leaf extract is the richest in phenolic compounds and flavonoids with a content of 523 ± 4.55 μg EAG / mg of Ext and 474 ± 2.18 μg EQ /mg of Ext respectively.The antioxidant activity was evaluated in vitro by the DPPH• test and the anti-hemolytic power assay. The obtained results show that the extract can act as free-radical scavengers at all concentrations.This antioxidant capacity is confirmed by the anti-hemolytic power test. Indeed, this extract showed a greater protective effect against erythrocyte hemolysis induced by H2O2.The evaluation of the antithrombotic activity of Cassia angustifolia was also evaluated in vitro by using the cephalin-kaolin time (TCK), prothrombin time (TQ) and fibrinogen test (FIB) and the thrombolytic power. The coagulation times obtained on normal plasma in the presence of this extract indicate that they exert an anticoagulant activity on the two coagulation pathways, but this activity is more marked on the endogenous pathway than on the exogenous pathway. Thus, this extract showed a good thrombolytic power against clots