Criblage de composés antivasculaires par le test sur la membrane chorioallantoïque (CAM)

Abstract

The fight against cancer by inhibiting tumor vascularization is based on two complementary approaches: preventing the formation of new blood vessels (anti-angiogenesis) and disrupting existing vessels (vascular disruption therapy). In this study, the antivasculare effects of crude Ginkgo biloba extract were investigated using the chorioallantoic membrane (CAM) model. On the 3ᵉ day of incubation, in ovo cultures were prepared. On the 8ᵉ day, the plant preparation was applied directly to the CAM. A saline solution served as the negative control, while dexamethasone and garlic juice served as the positive controls. Photographs were taken, allowing for the monitoring of the induced vascular alterations. Ginkgo biloba demonstrated a notable anti-angiogenic action by blocking neovascularization without altering existing vessels. It is distinguished by the absence of major adverse effects (such as irritation, bleeding, or thrombosis) on the CAM. However, it induces membrane keratinization, vascular vasoconstriction, a cessation of blood flow, and a decrease in vascular density, all without causing embryonic death.