Remède naturel contre les brûlures : contribution d’une plante du terroir algérien

Abstract

This study aims to analyze the biological characteristics of the hydroethanolic extract of Carthamus caeruleus L. roots. The extraction yield was 10.30%, indicating a successful extraction. Phytochemical analyses revealed a high concentration of polyphenols (42.308 ± 2.466 mg GAE/g) and flavonoids (57.625 ± 1.237 mg QE/g), as determined by spectrophotometric techniques. The antioxidant activity was assessed using the DPPH and ABTS assays. The results demonstrated significant free radical scavenging activity, with IC₅₀ values of 0,210±0,016 mg/mL (DPPH) and 0,093±0,001 mg/mL (ABTS), although these values were higher than those of ascorbic acid used as a reference standard. Antibacterial efficacy was evaluated using the well diffusion method against four bacterial strains: Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus. The extracts exhibited variable efficacy depending on the strain and the extraction method, with the hydro ethanolic extract showing the highest activity, particularly against Bacillus cereus (inhibition zone > 28 mm). Finally, the in vitro anti-inflammatory activity, based on the inhibition of albumin denaturation (BSA), indicated that the extract possesses moderate activity, with an IC₅₀ of 3,824±0,191 mg/mL, compared to diclofenac sodium (IC₅₀ = 0,072±0,017 mg/mL). Carthamus caeruleus L. demonstrates promising antioxidant, antibacterial, and anti-inflammatory properties, supporting its traditional use and offering potential for future medical applications.