QF-PCRبواسطة yتصميم عدة للكشف عن الحذف المجهري للكروموسوم

Abstract

Small deletions on the Y chromosome are among the important genetic alterations that may affect male fertility and sexual development. Since the Y chromosome carries information that governs sex and sexual evelopment, detection of small deletions is essential for the diagnosis and understanding of many sexual and health disorders.The aim of this note is to design a set of targeted primers to detect small deletions in the Y chromosome using quantitative fluorescent polymerase chain reaction (QF-PCR), which is one of the latest and most efficient techniques for analyzing genetic mutations, we identified target sites on the Y chromosome using available genetic data, including common deletion sites, and used these sites to design suitable primers. The designed primers are tested to determine their efficiency and accuracy in detecting specific deletions using scientific experiments, and the results are analyzed by fluorescent polymerase chain reaction (PCR) and gene analysis, and then the results are evaluated and compared with other techniques used for deletion detection, highlighting the efficiency and reliability of QF-PCR.The use of QF-PCR to detect small deletions on the Y chromosome represents an important step in the development of genetic diagnostic tools and understanding of the genetic effects of Y chromosome deletions and associated sexual and health disorders, and thus this designed set of primers may contribute significantly to improving early diagnosis and treatment of patients affected by these deletions.