Etude de l’effet irritant des substances chimiques sur les vaisseaux Sanguins de la membrane chorioallantoidienne (CAM).

Abstract

Background : The burden of cancer is a significant public health issue, andits treatment relies on surgery, radiotherapy, chemotherapy, hormone therapy and targeted therapies. Recently, new therapeutic strategies have emerged that target tumor vascularization using two different approaches. Anti-angiogenic therapy aims to inhibit the formation of new blood vessels from pre-existing ones. On the other hand, vascular disrupting agents (VDAs) work to collapse the established vascular network within tumors.Aim :This study investigates the anti-vascular effects of crude garlic juice (Allium sativum L.) on the blood vessels of the chorioallantoic embrane (CAM) in chicken embryos, both in ovo and ex ovo.Methods :On the third and fourth days of incubation (ED3 and ED4), in ovo and ex ovo cultures were prepared and then reincubated. On the eighth day (ED8), fresh garlic juice and its serial dilutions were prepared and applied to the CAM. Images were captured and the time it took for irritation to occur was recorded.Findings: The results show hemorrhagic events, coagulations (thromboses), appearance of hyperemia and dose- and time-dependent vasodilation. In addition, we recorded an interruption of blood flow and the appearance of ghost vessels. It seems that the crude garlic juice is not lethal to embryos.Conclusion: The crude extract of garlic (Allium sativum) produces a pronounced irritating effect on CAM blood vessels. This observation suggests that crude garlic juice may be recommended for further testing as a potential anti-cancer therapy targeting tumor vascularization through a VDT approach.